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DNA Molarity Formula:
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DNA molarity calculation converts DNA concentration from mass/volume units (g/L) to molar concentration (mol/L) using the molecular weight of the DNA. This is essential for molecular biology experiments where precise molar ratios are required.
The calculator uses the molarity formula:
Where:
Explanation: The formula converts mass concentration to molar concentration by dividing by the molecular weight of the DNA.
Details: Accurate molarity calculation is crucial for PCR, sequencing, cloning, and other molecular biology techniques where precise DNA amounts are required for successful reactions and reproducible results.
Tips: Enter DNA concentration in g/L and molecular weight in g/mol. Both values must be positive numbers. For typical calculations, DNA concentration is often provided in ng/μL or μg/mL, which may need conversion to g/L first.
Q1: How do I convert ng/μL to g/L?
A: Multiply ng/μL by 1,000 to get g/L (since 1 ng/μL = 1,000 g/L).
Q2: How do I determine DNA molecular weight?
A: For double-stranded DNA, average molecular weight is approximately 660 g/mol per base pair. For specific sequences, use online calculators that account for exact nucleotide composition.
Q3: Why is molarity important in molecular biology?
A: Molarity ensures consistent reaction conditions across experiments and allows for precise calculation of DNA:enzyme ratios, primer concentrations, and other critical reaction components.
Q4: Can this calculator be used for RNA?
A: Yes, the same formula applies to RNA molarity calculations, though the average molecular weight per base differs (approximately 340 g/mol per nucleotide for single-stranded RNA).
Q5: What if I have concentration in different units?
A: Convert to g/L first: μg/mL = g/L, ng/μL = 1000 g/L, mg/mL = 1000 g/L.