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Dilution Formula:
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Dilution calculation is a fundamental laboratory technique used to prepare solutions of desired concentration from stock solutions. The process involves adding solvent to decrease the concentration of a solute in a solution while maintaining the same amount of solute.
The calculator uses the dilution formula:
Where:
Explanation: The formula states that the product of initial concentration and volume equals the product of final concentration and volume, assuming the amount of solute remains constant.
Details: Accurate dilution calculations are essential in various scientific fields including chemistry, biology, pharmacology, and medical diagnostics. They ensure precise concentrations for experiments, drug formulations, and laboratory tests.
Tips: Enter the initial concentration and volume, desired final concentration, and select appropriate units. The calculator will determine the final volume needed and the dilution factor.
Q1: What is the difference between mg/mL and μg/mL?
A: mg/mL (milligrams per milliliter) is 1000 times larger than μg/mL (micrograms per milliliter). 1 mg/mL = 1000 μg/mL.
Q2: How do I convert between molarity (mM) and mass concentration?
A: To convert mM to mg/mL, multiply by the molecular weight and divide by 1000. The calculator uses a default molecular weight of 180.16 g/mol for conversion.
Q3: What is a dilution factor?
A: The dilution factor represents how many times the original solution was diluted. A 10x dilution means the solution was diluted 10 times.
Q4: Can I use this calculator for serial dilutions?
A: This calculator is designed for single-step dilutions. For serial dilutions, you would need to perform multiple calculations.
Q5: Why are my results slightly different from manual calculations?
A: The calculator uses precise conversion factors and rounding. Small differences may occur due to rounding conventions.